8/28/2023 0 Comments Blue planet deep sea brine sea![]() ![]() ![]() ![]() ![]() Moreover, NitraS-ATII showed higher thermal stability compared to a closely related nitrilase, in addition to its observed tolerance towards high concentrations of selected heavy metals. Characterization of the purified NitraS-ATII revealed its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids. The enzyme was cloned, expressed and purified. The isolated 338 amino-acid nitrilase (NitraS-ATII) is part of a highly conserved operon in different bacterial phyla with indiscernible function. We identified and isolated a nitrilase from the Atlantis II Deep Brine Pool in the Red Sea LCL. The Lower Convective Layer (LCL) of the Atlantis II Deep Brine Pool in the Red Sea is characterized by elevated temperature (68 ☌), high salt concentrations (250 ‰), anoxic conditions and high heavy metal concentrations. The Red Sea brine pools are characterized by multitude of extreme conditions. Extreme environments are potential habitats for the isolation and characterization of extremozymes including nitrilases with unique resistant properties. It’s absolutely amazing to see vast array of new species and the overall dense environment that surrounds this Underwater Lake.Nitrilases, which hydrolyze nitriles in a one-step reaction into carboxylic acids and ammonia, gained increasing attention because of the abundance of nitrile compounds in nature and their use in fine chemicals and pharmaceutics. The bacteria from the lake actually creates the entire environment around the Brine Lake, that actually ‘fixes’ the methane’s energy so that the mussels can use it ( UnderWater Lake Discovery). Just like Jurassic Park, ‘Life finds a way!'” ( Underwater Oceanic Lakes 64). Even small creatures called bug-bears (microscopic soil eaters that live in all dirt) can survive the vacuum of space if it were free of radiation. The creatures that survive in this micro ecosystem depend on the cold seep of methane from the lake and surrounding soil. Isolated and fragile the key to life here is in the bacteria that uses methane for energy. “This is like finding a lake in the desert or an oasis. So think about it this way, animals on land are always searching for food and water sources… it’s actually the exact, same thing with this “Lake”. This Underwater Lake must provide something to the life around it in order for it to be such a densely populated area. So now you have to ask yourself, what is important about this lake? How can it possibly be that there’s just mud along the ocean floor, barely anything, and then suddenly boom this spring of life out of nowhere. However, “due to lethal levels of hyper-salinity, only bacteria and other microorganisms are able to live within these underwater lakes” while a vast array of mollusks and crustaceans crowd its shores creating a rich, diverse, undersea environment, teaming with life ( Underwater Brine Lake). The thought, that an Undersea Brim Lake can exit is remarkable, especially knowing that it creates its own ecosystems around its shores. DeGruy and the crew actually were going to try and descend down into the Brime lake but the liquid was so unbelievably dense that the submersible literally bounced right off the surface, and sent little ripples/waves to the lake’s “shore”. Mike deGruy went down in a submersible for Blue Planet to help film the episode “The Deep” and that is when he got to see this “Underwater Lake”. In an interview DeGruy talks about how they didn’t really see much of anything at first- until “the most amazing thing… anywhere is when I went to the Gulf of Mexico, for Blue Planet, to film the Underwater Lake… And I’m like ‘Wait a minute! I’m already underwater! How can there be a lake?!’ But it was! …Fantasic area! Fantastic! I have no idea what’s in the lake…” (deGruy). What’s amazing about this environment is that the lake is made up of, a thick s alty brine – which is extremely dense. One of these is what is called an “ Underwater Lake” – something that you’d normally see in a Spongebob Squarepants episode actually exists at the bottom of our oceans. The world’s oceans are vastly unexplored and hold many creatures and environments that we didn’t think could possibly exist. ![]()
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